MicroRNA Expression Profiles in Human Samples and Cell Lines Revealed Nine miRNAs Associated with Cisplatin Resistance in High-Grade Serous Ovarian Cancer.

Metastasis and drug resistance are major contributors to cancer-related fatalities worldwide. In ovarian cancer (OC), a staggering 70% develop resistance to the front-line therapy, cisplatin. Despite proposed mechanisms, the molecular events driving cisplatin resistance remain unclear. Dysregulated microRNAs (miRNAs) play a role in OC initiation, progression, and chemoresistance, yet few studies have compared miRNA expression in OC samples and cell lines. This study aimed to identify key miRNAs involved in the cisplatin resistance of high-grade-serous-ovarian-cancer (HGSOC), the most common gynecological malignancy. MiRNA expression profiles were conducted on RNA isolated from formalin-fixed-paraffin-embedded human ovarian tumor samples and HGSOC cell lines. Nine miRNAs were identified in both sample types. Targeting these with oligonucleotide miRNA inhibitors (OMIs) reduced proliferation by more than 50% for miR-203a, miR-96-5p, miR-10a-5p, miR-141-3p, miR-200c-3p, miR-182-5p, miR-183-5p, and miR-1206. OMIs significantly reduced migration for miR-183-5p, miR-203a, miR-296-5p, and miR-1206. Molecular pathway analysis revealed that the nine miRNAs regulate pathways associated with proliferation, invasion, and chemoresistance through PTEN, ZEB1, FOXO1, and SNAI2. High expression of miR-1206, miR-10a-5p, miR-141-3p, and miR-96-5p correlated with poor prognosis in OC patients according to the KM plotter database. These nine miRNAs could be used as targets for therapy and as markers of cisplatin response.

Advances in current in vitro models on neurodegenerative diseases.

Many neurodegenerative diseases are identified but their causes and cure are far from being well-known. The problem resides in the complexity of the neural tissue and its location which hinders its easy evaluation. Although necessary in the drug discovery process, in vivo animal models need to be reduced and show relevant differences with the human tissues that guide scientists to inquire about other possible options which lead to in vitro models being explored. From organoids to organ-on-a-chips, 3D models are considered the cutting-edge technology in cell culture. Cell choice is a big parameter to take into consideration when planning an in vitro model and cells capable of mimicking both healthy and diseased tissue, such as induced pluripotent stem cells (iPSC), are recognized as good candidates. Hence, we present a critical review of the latest models used to study neurodegenerative disease, how these models have evolved introducing microfluidics platforms, 3D cell cultures, and the use of induced pluripotent cells to better mimic the neural tissue environment in pathological conditions.

Mexican Biobank advances population and medical genomics of diverse ancestries.

Latin America continues to be severely underrepresented in genomics research, and fine-scale genetic histories and complex trait architectures remain hidden owing to insufficient data1. To fill this gap, the Mexican Biobank project genotyped 6,057 individuals from 898 rural and urban localities across all 32 states in Mexico at a resolution of 1.8 million genome-wide markers with linked complex trait and disease information creating a valuable nationwide genotype-phenotype database. Here, using ancestry deconvolution and inference of identity-by-descent segments, we inferred ancestral population sizes across Mesoamerican regions over time, unravelling Indigenous, colonial and postcolonial demographic dynamics2-6. We observed variation in runs of homozygosity among genomic regions with different ancestries reflecting distinct demographic histories and, in turn, different distributions of rare deleterious variants. We conducted genome-wide association studies (GWAS) for 22 complex traits and found that several traits are better predicted using the Mexican Biobank GWAS compared to the UK Biobank GWAS7,8. We identified genetic and environmental factors associating with trait variation, such as the length of the genome in runs of homozygosity as a predictor for body mass index, triglycerides, glucose and height. This study provides insights into the genetic histories of individuals in Mexico and dissects their complex trait architectures, both crucial for making precision and preventive medicine initiatives accessible worldwide.

Automation, live-cell imaging, and endpoint cell viability for prostate cancer drug screens.

Androgen deprivation therapy (ADT) is the standard of care for high risk and advanced prostate cancer; however, disease progression from androgen-dependent prostate cancer (ADPC) to lethal and incurable castration-resistant prostate cancer (CRPC) and (in a substantial minority of cases) neuroendocrine prostate cancer (NEPC) is common. Identifying effective targeted therapies is challenging because of acquired resistance to established treatments and the vast heterogeneity of advanced prostate cancer (PC). To streamline the identification of potentially active prostate cancer therapeutics, we have developed an adaptable semi-automated protocol which optimizes cell growth and leverages automation to enhance robustness, reproducibility, and throughput while integrating live-cell imaging and endpoint viability assays to assess drug efficacy in vitro. In this study, culture conditions for 72-hr drug screens in 96-well plates were established for a large, representative panel of human prostate cell lines including: BPH-1 and RWPE-1 (non-tumorigenic), LNCaP and VCaP (ADPC), C4-2B and 22Rv1 (CRPC), DU 145 and PC3 (androgen receptor-null CRPC), and NCI-H660 (NEPC). The cell growth and 72-hr confluence for each cell line was optimized for real-time imaging and endpoint viability assays prior to screening for novel or repurposed drugs as proof of protocol validity. We demonstrated effectiveness and reliability of this pipeline through validation of the established finding that the first-in-class BET and CBP/p300 dual inhibitor EP-31670 is an effective compound in reducing ADPC and CRPC cell growth. In addition, we found that insulin-like growth factor-1 receptor (IGF-1R) inhibitor linsitinib is a potential pharmacological agent against highly lethal and drug-resistant NEPC NCI-H660 cells. This protocol can be employed across other cancer types and represents an adaptable strategy to optimize assay-specific cell growth conditions and simultaneously assess drug efficacy across multiple cell lines.

Inhibition of the serine/threonine kinase BUB1 reverses taxane resistance in prostate cancer.

Men with incurable castration resistant prostate cancer (CRPC) are typically treated with taxanes; however, drug resistance rapidly develops. We previously identified a clinically relevant seven gene network in aggressive CRPC, which includes the spindle assembly checkpoint (SAC) kinase BUB1. Since SAC is deregulated in taxane resistant PC, we evaluated BUB1 and found that it was over-expressed in advanced PC patient datasets and taxane resistant PC cells. Treatment with a specific BUB1 kinase inhibitor re-sensitized resistant CRPC cells, including cells expressing constitutively active androgen receptor (AR) variants, to clinically used taxanes. Consistent with a role of AR variants in taxane resistance, ectopically expressed AR-V7 increased BUB1 levels and reduced sensitivity to taxanes. This work shows that disruption of BUB1 kinase activity reverted resistance to taxanes, which is essential to advancing BUB1 as a potential therapeutic target for intractable chemotherapy resistant CRPC including AR variant driven CRPC, which lacks durable treatment options.

Gaining knowledge about biomarkers of the immune system and inflammation in the saliva of pigs: The case of myeloperoxidase, S100A12, and ITIH4.

An assay for the measurement of myeloperoxidase (Mpx) in porcine saliva was developed and validated, and factors influencing Mpx and another two biomarkers of inflammation and immune system, the protein S100A12 and the inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4), were studied. The spectrophotometric method for Mpx measurement validated in this assay showed an adequate analytical performance including precision and accuracy. When a group of twenty healthy pigs was sampled every 4 h from 8 a.m. until 8 p.m., Mpx and S100A12 showed significant increases at 4 p.m., whereas ITIH4 concentration showed a significant decrease at 12 a.m. Increases were also seen in salivary Mpx, S100A12, and ITIH4 levels 24 h after the intramuscular administration of Escherichia coli lipopolysaccharide in five pigs; whereas in a non-septic inflammation after the subcutaneous administration of turpentine oil to five pigs changes were seen in S100A12 at 3 h and in ITIH4 at 48 h. When a stressful situation consisting of the transportation and stay of 4 h to a slaughterhouse of 24 pigs was performed, all analytes were increased after 4 h of lairage in the slaughterhouse compared with the values that were obtained the day before at the same time of the day. Mpx can be measured in the saliva of pigs with the automated assay described in this report. Mpx, S100A12, and ITIH4 salivary levels can change depending on the hour of the day in which the sample is taken, and increases can be produced due to sepsis, non-septic inflammation and stress.

Relationship between child-to-parent violence and cumulative childhood adversity: the mediating role of parental attachment, resilience, and emotional intelligence.

Introduction: Recent research on aggressor profiles in child-to-parent violence (CPV) seems to provide promising results. However, this phenomenon has been poorly addressed in the adverse childhood experiences (ACEs) framework. This study aimed to explore the frequency of different types of ACEs and cumulative ACEs in adolescents who exert CPV, to analyze the differences between aggressors with different levels of cumulative ACEs in parental attachment, resilience, and emotional intelligence, and to evaluate the associations between these variables, as well as a possible mediational model. Methods: A total of 3,142 Spanish adolescents (50.7% girls) aged between 12 and 18 years from educational centers participated. Results: Adolescents who exerted CPV presented higher rates of ACEs both independently and cumulatively than those without CPV. Aggressors with cumulative ACEs (88%) in general presented more insecure parental attachment, lower resilience, and lower emotional intelligence than those without cumulative ACEs, and, in turn, aggressors with high levels of cumulative ACEs than those with low levels of cumulative ACEs. Significant associations were identified between CPV, ACEs, insecure parental attachment, resilience, and emotional intelligence. The mediation model suggested that ACEs are related to CPV through preoccupied and traumatized parental attachment and also through low levels of emotional intelligence. Discussion: The findings provide a better understanding of CPV from the perspective of ACEs, especially of those cases that involve an accumulation of adverse experiences during childhood, and suggest greater professional attention to these cases with the design of specialized CPV intervention programs.

Breastfeeding is associated with a delayed decrease in postprandial maternal glucose concentration.

BACKGROUND: Breastfeeding has long-term benefits in reducing the risk of diabetes; however, information about the acute influence on maternal glucose profile is scarce. Thus, the aim of the study was to assess maternal glucose fluctuations associated with breastfeeding episodes in women with normal glucose status. METHODS: We performed an observational study of glucose fluctuations with breastfeeding episodes in 26 women with normal glucose status in fasting and postprandial state. Continuous glucose monitoring was performed using CGMS MiniMed Gold®/iPro2® (Medtronic, Dublin, Ireland) three months after delivery under real-life conditions. We compared fasting and postprandial periods of 150 minutes affected or not by a breastfeeding episode. RESULTS: Mean glucose concentration of postprandial periods affected by breastfeeding was lower than not affected (-6.31 mg/dL [95% CI: -11.17, -1.62] P<0.01). Glucose concentration was significantly lower between 50 and 105 minutes after meal initiation (maximum difference -9.19 mg/dL [95% CI: -16.03, -2.16] at 91-95 min). Mean glucose concentrations of fasting periods affected by breastfeeding were similar to those not affected (-0.18 mg/dL [95% CI: -2.7, 0] P=0.831). CONCLUSIONS: In women with normal glucose status, breastfeeding episodes are associated with a lower glucose concentration in the postprandial but not in the fasting state.

Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells.

Introduction: Three-dimensional (3D) bioprinting is a promising technique for the development of neuronal in vitro models because it controls the deposition of materials and cells. Finding a biomaterial that supports neural differentiation in vitro while ensuring compatibility with the technique of 3D bioprinting of a self-standing construct is a challenge. Methods: In this study, gelatin methacryloyl (GelMA), methacrylated alginate (AlgMA), and hyaluronic acid (HA) were examined by exploiting their biocompatibility and tunable mechanical properties to resemble the extracellular matrix (ECM) and to create a suitable material for printing neural progenitor cells (NPCs), supporting their long-term differentiation. NPCs were printed and differentiated for up to 15 days, and cell viability and neuronal differentiation markers were assessed throughout the culture. Results and Discussion: This composite biomaterial presented the desired physical properties to mimic the ECM of the brain with high water intake, low stiffness, and slow degradation while allowing the printing of defined structures. The viability rates were maintained at approximately 80% at all time points. However, the levels of ß-III tubulin marker increased over time, demonstrating the compatibility of this biomaterial with neuronal cell culture and differentiation. Furthermore, these cells showed increased maturation with corresponding functional properties, which was also demonstrated by the formation of a neuronal network that was observed by recording spontaneous activity via Ca2+ imaging.

Risk Factors in Specialists and Generalists of Child-to-Parent Violence: Gender Differences and Predictors of Reactive and Proactive Reasons.

Recent research on child-to-parent violence (CPV) is advancing in the analysis of the specialist profile (aggressors who show only CPV) and the generalist profile (aggressors who show peer violence in addition to CPV). However, although differences have been found between girls and boys in the risk factors for CPV, there are no studies that analyze these differences according to the type of aggressor. Likewise, the importance of identifying the factors that differentially predict reactive and proactive CPV has been noted but has not been examined in different types of aggressors. The aims of this study were to examine gender differences in CPV patterns, emotional intelligence, parental victimization, and parental permissiveness and to analyze whether these variables predict reactive and proactive CPV, both according to aggressor type. A total of 1559 Spanish CPV aggressors (54.6% females) aged between 12 and 18 years from educational centers participated in the study (22.4% exercised only CPV (specialists) and 77.6% exercised peer violence in addition to CPV (generalists)). In general, no differences were found between girls and boys in the specialist profile, but differences were found in the generalist profile. Specifically, generalist girls exercised more psychological and control/domain violence toward mothers, while boys exercised more physical violence toward fathers and had more parental permissiveness. In specialists, parental victimization predicted reactive CPV, while parental permissiveness predicted proactive CPV. In contrast, in generalists, both parental victimization and parental permissiveness predicted both reactive and proactive CPV. Low emotional regulation was a significant predictor in both cases. This study identifies differences among girls and boys in CPV risk factors and among variables that predict reactive and proactive CPV and that these results differ between specialist and generalist aggressors. Implications for research and professional practice are discussed, highlighting the need to design and implement prevention and intervention programs specialized in the type of aggressor, paying special attention to gender differences and to the factors that motivate one or the other type of CPV.

High-Capacity Mesoporous Silica Nanocarriers of siRNA for Applications in Retinal Delivery.

The main cause of subretinal neovascularisation in wet age-related macular degeneration (AMD) is an abnormal expression in the retinal pigment epithelium (RPE) of the vascular endothelial growth factor (VEGF). Current approaches for the treatment of AMD present considerable issues that could be overcome by encapsulating anti-VEGF drugs in suitable nanocarriers, thus providing better penetration, higher retention times, and sustained release. In this work, the ability of large pore mesoporous silica nanoparticles (LP-MSNs) to transport and protect nucleic acid molecules is exploited to develop an innovative LP-MSN-based nanosystem for the topical administration of anti-VEGF siRNA molecules to RPE cells. siRNA is loaded into LP-MSN mesopores, while the external surface of the nanodevices is functionalised with polyethylenimine (PEI) chains that allow the controlled release of siRNA and promote endosomal escape to facilitate cytosolic delivery of the cargo. The successful results obtained for VEGF silencing in ARPE-19 RPE cells demonstrate that the designed nanodevice is suitable as an siRNA transporter.

Results of a strategy based on clinical observation of newborns at risk of early-onset neonatal sepsis.

BACKGROUND: Serial clinical observation of asymptomatic newborns at risk of early-onset sepsis is an alternative option for which there is limited scientific evidence. AIMS: To evaluate the rate of protocol compliance, the impact on blood tests, percentage of hospitalizations and subsequent procedures, and course of diagnosed early-onset sepsis cases of a protocol based on serial clinical observation. METHODS: Retrospective observational study comparing an 18-month period under this protocol against a previous protocol based on laboratory tests. SUBJECTS: 6895 asymptomatic newborns with over 35 weeks of gestation. OUTCOME MEASURES: number of evaluations performed on each subject at risk, percentage of patients undergoing blood draws and hospitalization rates. RESULTS: Some of the evaluations included in the protocol were omitted in 51.6 % of the newborns undergoing the physical examinations. The implementation of this new approach was associated with a decrease in the percentage of patients undergoing blood draws from 16.8 % to 0.7 % (p < 0.001) with no differences in the progression of the five cases of sepsis studied in each period. The serial clinical observation protocol was associated with a significant increase in hospitalizations for suspected infection, although with no difference in the rate of lumbar punctures performed or antibiotic treatments administered. CONCLUSION: Compliance with the serial clinical observation protocol can be difficult. This approach often detects newborns with abnormal clinical data that are not explained by early-onset sepsis. Clinical observation is a safe option that minimizes the rate of blood draws.

Child-to-parent Violence Offenders (Specialists vs. Generalists): The Role of Direct Victimization at Home / Menores con medidas judiciales por violencia filio-parental (especialistas vs. generalistas): el papel de la victimización directa en el hogar

Background/Objectives: Current research has identified direct victimization at home as one of the main predictors of child-to-parent violence (CPV). However, the mechanisms involved in this relationship have not been studied. Thus, we first analyze the differences between CPV offenders and offenders who have committed other types of offenses, as well as the differences between two types of CPV offenders: specialists (those who have only committed CPV) and generalists (those who have committed CPV in addition to other offenses), in multiple risk factors. Next, we specifically examine direct victimization at home and its relationship with social-cognitive processing in CPV. Method: The sample consisted of 208 Spanish young offenders recruited from the Juvenile Justice Service (163 males) aged 14-20 years. Concretely, 83 were CPV offenders, 126 other offenders and concerning the CPV group, 57 were specialists and 26 generalists. A wide range of individual, family, and social variables were evaluated. Results: Compared to other offenders, CPV offenders show more socio-cognitive difficulties, less parental warmth and more parental criticism/rejection, more direct victimization at home and more vicarious victimization at school and in the street. Specialist and generalist CPV offenders differ significantly in their characteristics, with the generalists showing a more negative profile than the specialists. (AU)

Antecedentes/Objetivos: La investigación actual ha identificado a la victimización directa en el hogar como uno de los principales predictores de la violencia filio-parental (VFP). Sin embargo, los mecanismos implicados en esta relación no han sido estudiados. Así, en el presente estudio analizamos, en primer lugar, las diferencias entre jóvenes infractores con delitos relacionados con la VFP y jóvenes infractores con otro tipo de delitos, así como las diferencias entre dos tipos de infractores: especialistas (los que han cometido sólo delitos relacionados con la VFP) y generalistas (los que han cometido delitos relacionados con la VFP y además otros delitos), en múltiples factores de riesgo. A continuación, examinamos específicamente la victimización directa en el hogar y su relación con el procesamiento socio-cognitivo en la VFP. Método: La muestra estuvo compuesta por 208 jóvenes infractores españoles reclutados en el Servicio de Justicia Juvenil (163 chicos) con edades comprendidas entre los 14 y los 20 años. Concretamente, 83 con delitos relacionados con la VFP (57 especialistas y 26 generalistas) y 126 con otros delitos. Se evaluó una amplia gama de variables individuales, familiares y sociales. Resultados: En comparación con otros infractores, los jóvenes con delitos relacionados con la VFP muestran más dificultades socio-cognitivas, menos calidez parental y más crítica/rechazo parental, más victimización directa en el hogar y más victimización vicaria en la escuela y en la calle. Los especialistas y generalistas difieren significativamente en sus características, mostrando los generalistas un perfil más negativo que los especialistas. (AU)

Unraveling the relationship between conditions of ultrasonic-assisted extraction of chia mucilage and viscosity.

BACKGROUND: The application of chia mucilage still remains restricted due to the difficulty in achieving high extraction yields. The effect of ultrasonic-assisted extraction (UAE) conditions (temperature, seed:water ratio and time) on the rheological properties of chia mucilage extracts and the relation to the proportion of translucent phase (TP) and opaque phase (OP) of the mucilage in the extract were evaluated. RESULTS: UAE allowed the efficient extraction of chia mucilage from chia seeds. The desired overall optimal combination to maximize both yield and apparent viscosity was achieved at a seed:water ratio 1:10, a temperature of 25.3 °C and 53.7 min extraction time; the optimal conditions to obtain the maximum yield and minimum apparent viscosity were a seed:water ratio close to 1:20, temperature of 48.8 °C and 208.4 min extraction time. CONCLUSION: The results obtained in the present work demonstrated that the differences in rheological properties of chia mucilage extracts are due to the extraction methods used. Therefore, it is possible to modulate the extraction conditions in order to obtain different characteristics of the mucilage, maintaining a high extraction yield. © 2022 Society of Chemical Industry.

Unique and Additive Effects of Family and School Victimization on Child-to-Parent Violence.

In the study of child-to-parent violence (CPV), the perspective of the victimized aggressor has not been analyzed in depth, and the impact of different contexts of victimization even less so. The aim of this study was to examine the unique and additive effects of family victimization (direct and vicarious) and school victimization (bullying and cyberbullying) on predicting CPV toward fathers and mothers. The sample included 3,142 adolescents aged 12-18 years (M age = 14.32) from schools in southern Spain. The participants completed the Child-to-Parent Violence Questionnaire adolescent version, the Violence Exposure Scale, and the European Bullying/Cyberbullying Intervention Project Questionnaires. The findings showed that independently, both family and school victimization predicted CPV, with direct family victimization being the best predictor, and that jointly, contributed to a significant improvement in prediction, explaining approximately 20% of CPV. Furthermore, a correspondence was found between the type of violence experienced and the type of violence perpetrated. It is necessary to study the profile of the victimized aggressor in CPV in order to design interventions adapted to the specific needs of this profile.

Profile of the Victimized Aggressors in Child-to-Parent Violence: Differences According to the Type of Victimization.

Background/Objective: One of the main predictors of child-to-parent violence (CPV) is childhood victimization. Recent research indicates the need to study different types of CPV aggressors. However, the distinctive characteristics of the profile of the victimized aggressor and whether these characteristics differ according to the type of victimization have not been yet analyzed. Were examined differences between four types of CPV aggressors: with family victimization, with school victimization, with polyvictimization, and without victimization experiences. Method: A total of 1,559 Spanish adolescents aged between 12 and 18 years participated. Results: Compared to nonvictimized aggressors, victimized aggressors generally exercise more reactive and instrumental CPV and show more insecure parental attachment and less emotional and coping competencies. Additionally, among the types of victimization, polyvictimized aggressors show worse adjustment compared to those with a unique type of victimization. There are also significant differences according to the gender of the aggressor; however, the interaction effect between the type of aggressor and gender is not significant. Conclusions: Considering the profile of the victimized aggressor and the type of victimization experienced in CPV can provide valuable empirical information for the approach of differential explanatory mechanisms and for the design of prevention and intervention strategies adapted to the needs of this profile.

Antecedentes/Objetivo: Uno de los principales predictores de la violencia filio-parental (VFP) es la victimización infantil. Investigaciones recientes señalan la necesidad de estudiar diferentes tipos de agresores en la VFP. Sin embargo, todavía no se han analizado las características distintivas del perfil del agresor victimizado y si estas características también difieren según el tipo de victimización. Se examinaron diferencias entre cuatro tipos de agresores de VFP: con victimización familiar, con victimización escolar, con polivictimización y sin experiencias de victimización. Método: Participaron 1.559 adolescentes españoles con edades comprendidas entre 12 y 18 años. Resultados: El agresor victimizado, respecto al no victimizado, ejerce más VFP reactiva e instrumental, muestra un apego parental más inseguro y menos habilidades emocionales y de afrontamiento. Por tipos de victimización, los agresores polivictimizados muestran peor ajuste respecto a aquellos con un único tipo de victimización. Se encuentran diferencias significativas según el género de los agresores, sin embargo, el efecto de interacción entre el tipo de agresor y el género no fue significativo. Conclusiones: Considerar en la VFP el perfil del agresor victimizado, así como el tipo de victimización experimentada, puede proporcionar información empírica valiosa tanto para el planteamiento de mecanismos explicativos diferenciales como para el diseño de estrategias de prevención e intervención adaptadas a las necesidades de este perfil.

Differential Profile of Specialist Aggressor versus Generalist Aggressor in Child-to-Parent Violence.

Research on violence in general highlights the need to differentiate between those aggressors who only show specialized violence in the family context and those who also show generalized violence in other contexts outside the family. However, in the phenomenon of child-to-parent violence (CPV), the distinctive characteristics of this profile have not been yet analyzed. The aim of this study was to identify the typology of specialist aggressor versus the typology of generalist aggressor and examine whether they differ in their characteristics. A total of 1559 CPV aggressors participated, with ages between 12 and 18 years, of whom 22.4% exerted violence only towards parents (specialist aggressors) and 77.6% also exerted violence towards peers (generalist aggressors). The results show that specialized violence and generalized violence seem to follow different patterns according to age. The generalists were characterized by a more negative profile than the specialists. Specifically, the former showed more CPV and for more reasons, both reactive and proactive. Regarding individual characteristics, they obtained lower levels of emotional intelligence and resilience. Concerning family characteristics, they presented higher levels of insecure parental attachment and parental violence (direct and observed). The predictive variables retained in the regression model represented approximately 16.4% of the variation in the type of aggressor. This study supports the classification based on the specificity versus generality of violence, as it was found that specialist and generalist CPV aggressors differ significantly in their characteristics. It is considered that the findings could help to identify the differential mechanisms through which both types of aggressors have developed CPV. Further analysis of this profile can be of great use for the design of intervention and prevention programs adapted to the needs of each typology.

Profile of the Victimized Aggressor in Child-to-Parent Violence: Differences According to the Type of Victimization / Perfil del agresor victimizado en la violencia filio-parental: diferencias según el tipo de victimización

ResumenAntecedentes/Objetivo: Uno de los principales predictores de la violencia filio-parental (VFP) es la victimización infantil. Investigaciones recientes señalan la necesidad de estu-diar diferentes tipos de agresores en la VFP. Sin embargo, todavía no se han analizado las características distintivas del perfil del agresor victimizado y si estas características tam-bién difieren según el tipo de victimización. Se examinaron diferencias entre cuatro tipos de agresores de VFP: con victimización familiar, con victimización escolar, con polivicti-mización y sin experiencias de victimización. Método: Participaron 1.559 adolescentes españoles con edades comprendidas entre 12 y 18 años. Resultados: El agresor victimiza-do, respecto al no victimizado, ejerce más VFP reactiva e instrumental, muestra un apego parental más inseguro y menos habilidades emocionales y de afrontamiento. Por tipos de victimización, los agresores polivictimizados muestran peor ajuste respecto a aquellos con un único tipo de victimización. Se encuentran diferencias significativas se-gún el género de los agresores; sin embargo, el efecto de interacción entre el tipo de agresor y el género no fue significativo. Conclusiones: Considerar en la VFP el perfil del agresor victimizado, así como el tipo de victimización experimentada, puede proporcio-nar información empírica valiosa, tanto para el planteamiento de mecanismos explicati-vos diferenciales como para el diseño de estrategias de prevención e intervención adap-tadas a las necesidades de este perfil.

AbstractBackground/Objective: One of the main predictors of child-to-parent violence (CPV) is childhood victimization. Recent research indicates the need to study different types of CPV aggressors. However, the distinctive characteristics of the profile of the victimized aggressor and whether these characteristics differ according to the type of victimization have not been yet analyzed. Were examined differences between four types of CPV ag-gressors: with family victimization, with school victimization, with polyvictimization, and without victimization experiences. Method: A total of 1,559 Spanish adolescents aged between 12 and 18 years participated. Results: Compared to nonvictimized aggres-sors, victimized aggressors generally exercise more reactive and instrumental CPV and show more insecure parental attachment and less emotional and coping competencies. Additionally, among the types of victimization, polyvictimized aggressors show worse adjustment compared to those with a unique type of victimization. There are also signi-ficant differences according to the gender of the aggressor; however, the interaction effect between the type of aggressor and gender is not significant. Conclusions: Conside-ring the profile of the victimized aggressor and the type of victimization experienced in CPV can provide valuable empirical information for the approach of differential explana-tory mechanisms and for the design of prevention and intervention strategies adapted to the needs of this profile.

A de novo transcriptional atlas in Danaus plexippus reveals variability in dosage compensation across tissues.

A detailed knowledge of gene function in the monarch butterfly is still lacking. Here we generate a genome assembly from a Mexican nonmigratory population and used RNA-seq data from 14 biological samples for gene annotation and to construct an atlas portraying the breadth of gene expression during most of the monarch life cycle. Two thirds of the genes show expression changes, with long noncoding RNAs being particularly finely regulated during adulthood, and male-biased expression being four times more common than female-biased. The two portions of the monarch heterochromosome Z, one ancestral to the Lepidoptera and the other resulting from a chromosomal fusion, display distinct association with sex-biased expression, reflecting sample-dependent incompleteness or absence of dosage compensation in the ancestral but not the novel portion of the Z. This study presents extended genomic and transcriptomic resources that will facilitate a better understanding of the monarch's adaptation to a changing environment.

Tumor de células de Sertoli-Leydig: a propósito de un caso y revisión de la literatura reciente / Ovarian Sertoli-Leydig cell tumour: a clinical case and recent basic literature review

INTRODUCCIÓN: Los tumores de células de Sertoli-Leydig son neoplasias de ovario infrecuentes, lo que dificulta su diagnóstico y tratamiento. OBJETIVO: Revisar y sintetizar el manejo actual de los tumores de células de Sertoli-Leydig. MÉTODO: Se realizó una revisión de la literatura reciente sobre tumores de células de Sertoli-Leydig, a propósito de un caso en nuestro centro. RESULTADOS: Los tumores de las células de Sertoli-Leydig son infrecuentes, con mayor incidencia en edades tempranas. Ante una paciente joven con una lesión anexial unilateral y signos de virilización deberán considerarse estos tumores dentro del diagnóstico diferencial. En los estadios iniciales y en pacientes jóvenes podrá plantearse un tratamiento quirúrgico que preserve la fertilidad, y la asociación de tratamiento adyuvante dependerá de la diferenciación y del estadiaje del tumor.

INTRODUCTION: Sertoli-Leydig cell tumors are infrequent ovarian neoplasms, which difficults their diagnosis and treatment. Objective: To review and synthesize the current management of the Sertoli-Leydig cell tumor. METHOD: A review of the recent literature regarding the Sertoli-Leydig cell tumor was carried out, regarding a case in our center. RESULTS: Sertoli-Leydig cell tumors are an infrequent entity, with a higher incidence in early ages. In a young patient with a unilateral adnexal lesion and signs of virilization, these tumors should be considered within the differential diagnosis. In early stages and young patients, a surgical treatment that preserves fertility may be considered, and the association of adjuvant treatment will depend on the differentiation and staging of the tumor.